Modified Filter-Transfer-Freeze ("FTF") Technique for Raman Microspectroscopic Analysis of Single Cells V.2

Gordon T. Taylor; Zhuo-Qun "Emma" Li; Elizabeth Suter; Stephanie Chow

doi:10.17504/protocols.io.ikqccvw

Modified Filter-Transfer-Freeze ("FTF") Technique for Raman Microspectroscopic Analysis of Single Cells V.2

Gordon T. Taylor, Zhuo-Qun "Emma" Li, Elizabeth Suter, Stephanie Chow

Biology, Chemistry, Environmental Studies

Stony Brook University

Research & Scholarship: Other contribution

Abstract

 Published Protocol Version 2
    A method for concentrating aquatic microbes for single-cell analysis by Raman microspectroscopy. Modified from Hewes and Holm-Hansen (1983): A Method for Recovering Nanoplankton from Filters for Identification with the Microscope: The Filter-Transfer-Freeze (FTF) Technique. Limnology and Oceanography. 28(2): 389-394.
 
      Many materials commonly used in microscopy to examine individual microorganisms contribute contaminating Raman scattered emissions, e.g., most types of membranes and filters, glass slides, cover slips, immersion oil, glycerol, etc. and are therefore unsuitable for this application. We developed a protocol that enables single-cell analysis while minimizing extraneous Raman scattered photons.  The technique transfers cells from popular polycarbonate membranes to mirror-finished 304 stainless steel slides (1x3x0.0235') supplied by Stainless Supply® (Monroe, NC USA).

Original language	American English
Type	Protocol
DOIs	https://doi.org/10.17504/protocols.io.ikqccvw
State	Published - Jun 26 2017

Keywords

raman microspectroscopy
slide preparation
single-cell analysis
flourescent in situ hybridization

Disciplines

Life Sciences

Access to Document

10.17504/protocols.io.ikqccvw

Cite this

@misc{2eacae9b0aa84c03a02c38f7c81f8d77,

title = "Modified Filter-Transfer-Freeze ({"}FTF{"}) Technique for Raman Microspectroscopic Analysis of Single Cells V.2",

abstract = " Published Protocol Version 2 A method for concentrating aquatic microbes for single-cell analysis by Raman microspectroscopy. Modified from Hewes and Holm-Hansen (1983): A Method for Recovering Nanoplankton from Filters for Identification with the Microscope: The Filter-Transfer-Freeze (FTF) Technique. Limnology and Oceanography. 28(2): 389-394. Many materials commonly used in microscopy to examine individual microorganisms contribute contaminating Raman scattered emissions, e.g., most types of membranes and filters, glass slides, cover slips, immersion oil, glycerol, etc. and are therefore unsuitable for this application. We developed a protocol that enables single-cell analysis while minimizing extraneous Raman scattered photons. The technique transfers cells from popular polycarbonate membranes to mirror-finished 304 stainless steel slides (1x3x0.0235') supplied by Stainless Supply{\textregistered} (Monroe, NC USA).",

keywords = "raman microspectroscopy, slide preparation, single-cell analysis, flourescent in situ hybridization",

author = "Taylor, {Gordon T.} and Li, {Zhuo-Qun {"}Emma{"}} and Elizabeth Suter and Stephanie Chow",

note = "A method for concentrating aquatic microbes for single-cell analysis by Raman microspectroscopy. Modified from Hewes and Holm-Hansen (1983): A Method for Recovering Nanoplankton... Gordon T. Taylor, Zhuo-Qun {"}Emma{"} Li, Elizabeth Suter, Stephanie Chow (2017). Modified Filter-Transfer-Freeze ({"}FTF{"}) Technique for Raman Microspectroscopic Analysis of Single Cells. protocols.io dx.doi.org/10.17504/protocols.io.ikqccvw",

year = "2017",

month = jun,

day = "26",

doi = "10.17504/protocols.io.ikqccvw",

language = "American English",

type = "Other",

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TY - GEN

T1 - Modified Filter-Transfer-Freeze ("FTF") Technique for Raman Microspectroscopic Analysis of Single Cells V.2

AU - Taylor, Gordon T.

AU - Li, Zhuo-Qun "Emma"

AU - Suter, Elizabeth

AU - Chow, Stephanie

N1 - A method for concentrating aquatic microbes for single-cell analysis by Raman microspectroscopy. Modified from Hewes and Holm-Hansen (1983): A Method for Recovering Nanoplankton... Gordon T. Taylor, Zhuo-Qun "Emma" Li, Elizabeth Suter, Stephanie Chow (2017). Modified Filter-Transfer-Freeze ("FTF") Technique for Raman Microspectroscopic Analysis of Single Cells. protocols.io dx.doi.org/10.17504/protocols.io.ikqccvw

PY - 2017/6/26

Y1 - 2017/6/26

N2 - Published Protocol Version 2 A method for concentrating aquatic microbes for single-cell analysis by Raman microspectroscopy. Modified from Hewes and Holm-Hansen (1983): A Method for Recovering Nanoplankton from Filters for Identification with the Microscope: The Filter-Transfer-Freeze (FTF) Technique. Limnology and Oceanography. 28(2): 389-394. Many materials commonly used in microscopy to examine individual microorganisms contribute contaminating Raman scattered emissions, e.g., most types of membranes and filters, glass slides, cover slips, immersion oil, glycerol, etc. and are therefore unsuitable for this application. We developed a protocol that enables single-cell analysis while minimizing extraneous Raman scattered photons. The technique transfers cells from popular polycarbonate membranes to mirror-finished 304 stainless steel slides (1x3x0.0235') supplied by Stainless Supply® (Monroe, NC USA).

AB - Published Protocol Version 2 A method for concentrating aquatic microbes for single-cell analysis by Raman microspectroscopy. Modified from Hewes and Holm-Hansen (1983): A Method for Recovering Nanoplankton from Filters for Identification with the Microscope: The Filter-Transfer-Freeze (FTF) Technique. Limnology and Oceanography. 28(2): 389-394. Many materials commonly used in microscopy to examine individual microorganisms contribute contaminating Raman scattered emissions, e.g., most types of membranes and filters, glass slides, cover slips, immersion oil, glycerol, etc. and are therefore unsuitable for this application. We developed a protocol that enables single-cell analysis while minimizing extraneous Raman scattered photons. The technique transfers cells from popular polycarbonate membranes to mirror-finished 304 stainless steel slides (1x3x0.0235') supplied by Stainless Supply® (Monroe, NC USA).

KW - raman microspectroscopy

KW - slide preparation

KW - single-cell analysis

KW - flourescent in situ hybridization

U2 - 10.17504/protocols.io.ikqccvw

DO - 10.17504/protocols.io.ikqccvw

M3 - Other contribution

ER -